BoardION: real-time monitoring of Oxford Nanopore sequencing instruments
نویسندگان
چکیده
منابع مشابه
Oxford Nanopore MinION Sequencing and Genome Assembly
The revolution of genome sequencing is continuing after the successful second-generation sequencing (SGS) technology. The third-generation sequencing (TGS) technology, led by Pacific Biosciences (PacBio), is progressing rapidly, moving from a technology once only capable of providing data for small genome analysis, or for performing targeted screening, to one that promises high quality de novo ...
متن کاملChallenges in Transcriptome Applications Using Oxford Nanopore Sequencing Technology
1School of Biological Sciences, Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln NE 68588, USA 2Department of Computer Science and Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA *Corresponding author: Kan Liu, School of Biological Sciences, Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln NE 68588, USA, Tel: +1 402-472...
متن کاملNanocall: an open source basecaller for Oxford Nanopore sequencing data
MOTIVATION The highly portable Oxford Nanopore MinION sequencer has enabled new applications of genome sequencing directly in the field. However, the MinION currently relies on a cloud computing platform, Metrichor (metrichor.com), for translating locally generated sequencing data into basecalls. RESULTS To allow offline and private analysis of MinION data, we created Nanocall. Nanocall is th...
متن کاملComparison of Real Time Nanoparticle Monitoring Instruments in the Workplaces
BACKGROUND Relationships among portable scanning mobility particle sizer (P-SMPS), condensation particle counter (CPC), and surface area monitor (SAM), which are different metric measurement devices, were investigated, and two widely used research grade (RG)-SMPSs were compared to harmonize the measurement protocols. METHODS Pearson correlation analysis was performed to compare the relation b...
متن کاملBenchmarking of the Oxford Nanopore MinION sequencing for quantitative and qualitative assessment of cDNA populations
To assess the performance of the Oxford Nanopore Technologies MinION sequencing platform, cDNAs from the External RNA Controls Consortium (ERCC) RNA Spike-In mix were sequenced. This mix mimics mammalian mRNA species and consists of 92 polyadenylated transcripts with known concentration. cDNA libraries were generated using a template switching protocol to facilitate the direct comparison betwee...
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ژورنال
عنوان ژورنال: BMC Bioinformatics
سال: 2021
ISSN: 1471-2105
DOI: 10.1186/s12859-021-04161-0